| First Author | Waisman A | Year | 1996 |
| Journal | Lupus | Volume | 5 |
| Issue | 4 | Pages | 279-87 |
| PubMed ID | 8869899 | Mgi Jnum | J:40893 |
| Mgi Id | MGI:892607 | Doi | 10.1177/096120339600500407 |
| Citation | Waisman A, et al. (1996) Variable regions of two murine antibodies that bind the SLE associated 16/6 idiotype. Lupus 5(4):279-87 |
| abstractText | Experimental systemic lupus erythematosus can be induced in naive mice of different strains using a human monoclonal antibody bearing the 16/6 idiotype and a murine anti-16/6 Id monoclonal antibody designated 1A3-2. Herein we report the isolation of a second anti-16/6 Id antibody, 3F7-8, from BALB/c mice afflicted with experimental SLE. In contrast to the previously reported (1A3-2) anti-16/6 Id monoclonal antibody, mAb 3F7-8 does not induce experimental SLE upon immunization. The variable heavy and light chains of both antibodies were cloned and their sequences were determined. The VH of mAb 1A3-2 was found to express a germ line gene from the Q52 family, with a high homology to an anti-lysozyme antibody. The VH of monoclonal antibody 3F7-8 was found to express a 7183 germ line gene, showing over 95% homology with the VH of 12 anti-Sm antibodies isolated from MRL-lpr mice. Based on sequence homology to other known antibodies, we further demonstrated that monoclonal antibodies 1A3-2 and 3F7-8 bind lysozyme and the Sm ribonucleoproteins, respectively, in addition to their binding to the 16/6 Id. |
