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Allele : Col1a1<tm5(tetO-Pou5f1,-Klf4,-Myc)Jae> collagen, type I, alpha 1; targeted mutation 5, Rudolf Jaenisch
Primary Identifier  MGI:4430600 Allele Type  Targeted
Attribute String  Inducible, Inserted expressed sequence, RMCE-ready Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  RMCE on KH2 ES cells inserted a cassette containing (from 5' to 3') a loxP-flanked pgkNEOpolyA cassette, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 3F2A, and SV40 intron plus polyA signal into the 3' UTR. The 3F2A cassette contains the three mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by two different viral 2A oligopeptides that mediate ribosomal skipping (T2A [from insect Thosea asigna virus] and E2A [equine rhinitis A virus], respectively).
  • mutations:
  • Insertion
  • synonyms:
  • Col1a1-3F2A,
  • Col1a1<tetO-3F2A>,
  • Col1a1 3F2A,
  • Col1a1::tetO-3F2A,
  • Col1a1<3F2A>,
  • Col1a1-3F2A,
  • Col1a1<tetO-3F2A>,
  • Col1a1 3F2A,
  • Col1a1<3F2A>,
  • Col1a1::tetO-3F2A
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1 Feature

Trail: Allele

Genome

4 Expresses

Trail: Allele

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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1 Carried By

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0 Driven By

4 Publication categories

Trail: Allele




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