| First Author | Noda T | Year | 2006 |
| Journal | J Biotechnol | Volume | 126 |
| Issue | 2 | Pages | 230-6 |
| PubMed ID | 16730830 | Mgi Jnum | J:172992 |
| Mgi Id | MGI:5009405 | Doi | 10.1016/j.jbiotec.2006.04.021 |
| Citation | Noda T, et al. (2006) Transduction of NeuroD2 protein induced neural cell differentiation. J Biotechnol 126(2):230-6 |
| abstractText | NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta100-115) (lack of NLS), ND2(Delta123-134) (lack of basic) and ND2(Delta100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein. |
