| First Author | Morita T | Year | 1985 |
| Journal | Immunogenetics | Volume | 21 |
| Issue | 4 | Pages | 367-83 |
| PubMed ID | 3838967 | Mgi Jnum | J:50657 |
| Mgi Id | MGI:1309422 | Doi | 10.1007/BF00430802 |
| Citation | Morita T, et al. (1985) An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene. Immunogenetics 21(4):367-83 |
| abstractText | Two recombinant mice have been recovered from the progeny of Ttf/tw32 + animals. They have lost the tw32 lethality factor(s) and gained tufted, presumably from the T chromosome. Southern blot analysis of class I genes of these two new partial tPA027 and tPA286 haplotypes indicates that they have retained at least part of the major histocompatibility complex of the tw32 chromosome (H-2 haplotype H-2w28). We have prepared a phage library of Eco RI-digested DNA from homozygous tPA027 animals. Upon screening the library with a cDNA probe specific for H-2K genes, we isolated a class I gene displaying all of the distinctive features of a genuine H-2K gene, and which could thus be defined as an H-2Kw28 gene. The H-2Kw28 gene is 92-95% homologous to H-2Kb and H-2Kd genes and differs significantly from the other class I genes sequenced so far. Homology with the H-2Kb sequence reaches nearly 100% in the 3' part of the H-2Kw28 gene. Moreover, the homology with an H-2Kq cDNA sequence reaches 99.8%. Several hypotheses can account for the near identity of H-2Kb, H-2Kq, and H-2Kw28 gene sequences: either recombination between H-2w28 and H-2b and H-2q sequences occurred before or at the time the strain was established, or the class I genes of the tw32 chromosome and the H-2b and H-2q genes found in inbred strains of mice have separated from each other rather recently. |
