| First Author | Reyes AA | Year | 1982 |
| Journal | Proc Natl Acad Sci U S A | Volume | 79 |
| Issue | 10 | Pages | 3270-4 |
| PubMed ID | 6954478 | Mgi Jnum | J:50659 |
| Mgi Id | MGI:1309540 | Doi | 10.1073/pnas.79.10.3270 |
| Citation | Reyes AA, et al. (1982) Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc Natl Acad Sci U S A 79(10):3270-4 |
| abstractText | A library of cloned cDNAs constructed from the poly(A)+RNA of the murine thymoma cell line EL4 (b haplotype) was screened with a probe encoding a short region of the H-2Kb transplantation antigen. One of the clones isolated, pH202, contains a region that can code for a transplantation antigen with an amino acid sequence 98% homologous to that previously published for H-2Kb. Based on this high degree of homology, pH202 appears to encode the H-2Kb antigen from amino acid 66 through the carboxy terminus, including 386 nucleotides of 3'-untranslated sequence. The amino acid sequence deduced from pH202 suggests that the H-2Kb antigen is actually 2 amino acids longer than previously reported (a total of 348 residues). Four other differences in amino acid assignments are seen. Analysis of the DNA sequences of pH202 and other H-2 clones previously described in the literature suggests that alternative routes of splicing at the 3' end of the coding region are involved in the production of different transplantation antigen mRNAs. |
