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Publication : Molecular cloning and sequencing of H-2Kk cDNA: comparison with other H-2 genes and evidence for alternative splicing.

First Author  Reddy ES Year  1985
Journal  Gene Volume  38
Issue  1-3 Pages  239-44
PubMed ID  3840763 Mgi Jnum  J:68860
Mgi Id  MGI:1933553 Doi  10.1016/0378-1119(85)90223-9
Citation  Reddy ES, et al. (1985) Molecular cloning and sequencing of H-2Kk cDNA: comparison with other H-2 genes and evidence for alternative splicing. Gene 38(1-3):239-44
abstractText  A cDNA library was constructed from mouse L cells that were transfected with a human HLA-B7 gene fragment lacking the 5'-segment of exon 2 and all upstream sequences. A cDNA clone (pESP-C103) which is of mouse origin was detected by hybridization with the HLA-B7 gene. Comparison of the amino acid (aa) sequence predicted from the nucleotide sequence of the cDNA and the partial aa sequence of the H-2Kk antigen suggests that this cDNA (pESP-C103) codes for the H-2Kk antigen. This cDNA clone extends to the middle of the leader sequence. Comparison of the nucleotide and deduced aa sequences with those of other H-2 genes revealed an alternative splicing in exon 8 in the case of the H-2Kk gene.
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