| First Author | Forrest GL | Year | 1990 |
| Journal | Biochim Biophys Acta | Volume | 1048 |
| Issue | 2-3 | Pages | 149-55 |
| PubMed ID | 2182121 | Mgi Jnum | J:16350 |
| Mgi Id | MGI:64431 | Doi | 10.1016/0167-4781(90)90050-c |
| Citation | Forrest GL, et al. (1990) Induction of a human carbonyl reductase gene located on chromosome 21. Biochim Biophys Acta 1048(2-3):149-55 |
| abstractText | Carbonyl reductase (EC 1.1.1.184) belongs to the group of enzymes called aldo-keto reductases. It is a NADPH-dependent cytosolic protein with specificity for many carbonyl compounds including the antitumor anthracycline antibiotics, daunorubicin and doxorubicin. Human carbonyl reductase was cloned from a breast cancer cell line (MCF-7). The cDNA clone contained 1219 base paires with an open reading frame corresponding to 277 amino acids encoding a protein of Mr 30,375. Southern analysis of genomic DNA digested with several restriction enzymes and analyzed by hybridization with a labeled cDNA probe indicated that carbonyl reductase is probably coded by a single gene and does not belong to a family of structurally similar enzymes. Southern analysis of 17 mouse/human somatic cell hybrids showed that carbonyl reductase is located on chromosome 21. Carbonyl reductase mRNA could be induced 3-4-fold in 24 h with 10 microM 2,(3)-t-butyl-4-hydroxyanisole (BHA), beta-naphthoflavone or Sudan 1. |
