| First Author | Silkov A | Year | 2002 |
| Journal | J Biol Chem | Volume | 277 |
| Issue | 20 | Pages | 17821-9 |
| PubMed ID | 11856754 | Mgi Jnum | J:76571 |
| Mgi Id | MGI:2179684 | Doi | 10.1074/jbc.M200696200 |
| Citation | Silkov A, et al. (2002) Enhanced Apoptosis of B and T Lymphocytes in TAFII105 Dominant-negative Transgenic Mice Is Linked to Nuclear Factor-kappa B. J Biol Chem 277(20):17821-9 |
| abstractText | The general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 12-14 TBP-associated factors (TAF(II)s). Some TAF(II)s act as bridges between transcription activators and the general transcription machinery through direct interaction with activation domains. Although TAF-mediated transcription activation has been established, there is little genetic evidence connecting it to binding of an activator. TAF(II)105 is a substoichiometric subunit of transcription factor IID highly expressed in B lymphocytes. In this study, we examined the physiological role of TAF(II)105 and its mechanism of action in vivo by expressing two forms of dominant-negative mutant TAF(II)105 in mice. We show that TAF(II)105 has a pro-survival role in B and T lymphocytes, where the native protein is expressed. In addition, TAF(II)105 is important for T cell maturation and for production of certain antibody isotypes. These phenotypic alterations were absent in mice expressing a dominant-negative mutant that lacks one of the domains mediating p65/RelA binding in vitro. These findings provide support to the notion that interaction between the activator and TAF is important for their function in vivo. |
