| First Author | Greenbaum MP | Year | 2007 |
| Journal | Dev Biol | Volume | 305 |
| Issue | 2 | Pages | 389-96 |
| PubMed ID | 17383626 | Mgi Jnum | J:183221 |
| Mgi Id | MGI:5318028 | Doi | 10.1016/j.ydbio.2007.02.025 |
| Citation | Greenbaum MP, et al. (2007) Conversion of midbodies into germ cell intercellular bridges. Dev Biol 305(2):389-96 |
| abstractText | Whereas somatic cell cytokinesis resolves with abscission of the midbody, resulting in independent daughter cells, germ cell cytokinesis concludes with the formation of a stable intercellular bridge interconnecting daughter cells in a syncytium. While many proteins essential for abscission have been discovered, until recently, no proteins essential for mammalian germ cell intercellular bridge formation have been identified. Using TEX14 as a marker for the germ cell intercellular bridge, we show that TEX14 co-localizes with the centralspindlin complex, mitotic kinesin-like protein 1 (MKLP1) and male germ cell Rac GTPase-activating protein (MgcRacGAP) and converts these midbody matrix proteins into stable intercellular bridge components. In contrast, septins (SEPT) 2, 7 and 9 are transitional proteins in the newly forming bridge. In cultured somatic cells, TEX14 can localize to the midbody in the absence of other germ cell-specific factors, suggesting that TEX14 serves to bridge the somatic cytokinesis machinery to other germ cell proteins to form a stable intercellular bridge essential for male reproduction. |
