| First Author | Takakura H | Year | 1997 |
| Journal | Int Arch Allergy Immunol | Volume | 113 |
| Issue | 4 | Pages | 409-16 |
| PubMed ID | 9250586 | Mgi Jnum | J:43346 |
| Mgi Id | MGI:1097526 | Doi | 10.1159/000237616 |
| Citation | Takakura H, et al. (1997) Molecular cloning of caprine IL-6 cDNA and its expression in insect cells. Int Arch Allergy Immunol 113(4):409-16 |
| abstractText | To analyze the interactions among immunoneuroendocrinological systems in a miniature Shiba goat, we attempted to clone and express caprine cDNA-encoding interleukin-6 (IL-6) which is well-known to exert a variety of effects on neuroendocrinological functions in small experimental animals. The cDNA-encoding caprine IL-6 was molecularly cloned from a lipopolysaccharide-stimulated adherent splenocyte library by the reverse transcription-polymerase chain reaction. The sequence analysis shows that the cloned caprine IL-6 cDNA encodes a precursor protein of 208 amino acids that is processed to mature protein of 180 amino acids with a predicted molecular weight of 20,524. The amino acid sequence of caprine IL-6 displays 97.8 and 51.1% degree of homology with ovine and human equivalents, respectively. A recombinant baculovirus containing the caprine IL-6 cDNA was shown to induce insect cells to efficiently secrete caprine IL-6 into culture supernatant up to 10(5) U/ml, as confirmed by Western blot analysis and bioassay with an IL-6-dependent cell line. |
