Primary Identifier | MGI:6474233 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sinhcaf |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted in intron 1 and an FRT-flanked IRES-Betageo and second loxP site were inserted in intron 4. Cre-mediated recombination removed exons 1-4 and the Betageo. RT-qPCR and Western blot analysis confirmed the absence of transcript and protein in embryos. |