| First Author | Hoelsbrekken SE | Year | 2003 |
| Journal | J Immunol | Volume | 170 |
| Issue | 5 | Pages | 2259-63 |
| PubMed ID | 12594244 | Mgi Jnum | J:82012 |
| Mgi Id | MGI:2450510 | Doi | 10.4049/jimmunol.170.5.2259 |
| Citation | Hoelsbrekken SE, et al. (2003) Cutting edge: molecular cloning of a killer cell Ig-like receptor in the mouse and rat. J Immunol 170(5):2259-63 |
| abstractText | We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family. |
