Primary Identifier | MGI:3583817 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter, Transactivator | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. |