Primary Identifier | MGI:3054787 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Nt5e |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 2 and 3, encoding the presumptive catalyitic site, were replaced with a neomycin resistance gene. RT-PCR demonstrated a lack of transcript in mutants. Immunoperoxidase labeling against the endogenous protein confirmed its absence in kidneys. |