Primary Identifier | MGI:2388346 | Allele Type | Targeted |
Gene | Epor | Transmission | Germline |
Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The gene was truncated by replacement of exon 8 sequences encoding the distal half of the cytoplasmic domain with a neomycin resistance cassette. A tyrosine to phenylalanine substitution at residue 343 (Y343F) was also introduced. Expression of the mutant allele was confirmed by RT-PCR analysis of bone marrow RNA from homozygous mutant animals. Sequence analysis of RT-PCR products verified the presence of the Y343F amino acid substitution. |