First Author | Pan HA | Year | 2008 |
Journal | Fertil Steril | Volume | 89 |
Issue | 5 Suppl | Pages | 1324-7 |
PubMed ID | 17761180 | Mgi Jnum | J:136904 |
Mgi Id | MGI:3797325 | Doi | 10.1016/j.fertnstert.2007.03.041 |
Citation | Pan HA, et al. (2008) DAZL protein expression in mouse preimplantation embryo. Fertil Steril 89(5 Suppl):1324-7 |
abstractText | OBJECTIVE: To evaluate the expression pattern of Dazl (deleted in azoospermia-like) protein in the mouse preimplantation embryo. DESIGN: Experimental study. SETTING: Medical research laboratory in a university hospital. ANIMAL(S): Twenty female 28- to 35-day-old FVB mice. INTERVENTION(S): Embryo collection at 1.5, 2.5, and 3.5 days postcoitus (plug date, 0.5 d postcoitus) to examine the Dazl protein expression from the two-cell embryo to the blastocyst. MAIN OUTCOME MEASURE(S): Dazl protein expression was analyzed by immunofluorescent staining. RESULT(S): There is abundant expression of Dazl protein in the cytoplasm of the blastomere. Strong fluorescent signals of Dazl protein expression were found in preimplantation embryo cytoplasm, including two-cell, eight-cell, morula, and blastocyst. CONCLUSION(S): By using an antibody raised against mouse Daz-like protein (Dazl), we showed that Dazl protein is present in all cleaving stages of the preimplantation embryo. This is the first report on the protein expression of a Dazl gene during embryogenesis in mice. However, further study is needed to evaluate the molecular functional role of Dazl. |