| First Author | Smith KM | Year | 1998 |
| Journal | J Immunol | Volume | 161 |
| Issue | 1 | Pages | 7-10 |
| PubMed ID | 9647200 | Mgi Jnum | J:49203 |
| Mgi Id | MGI:1276991 | Doi | 10.4049/jimmunol.161.1.7 |
| Citation | Smith KM, et al. (1998) Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors. J Immunol 161(1):7-10 |
| abstractText | Several members of the Ly-49 receptor family inhibit NK cell-mediated lysis of targets expressing appropriate MHC class I molecules. Ly-49D and Ly-49H, two Ly-49 molecules that lack immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, associate with mouse DAP12, a molecule that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Cotransfection of either Ly-49D or Ly-49H with DAP12 induces surface expression of both Ly-49 and DAP12. The Ly-49/DAP12 complex was coimmunoprecipitated from the transfected cells, demonstrating a physical association of DAP12 with Ly-49D or Ly-49H in the plasma membrane. Stimulation of transfectants with Abs recognizing either Ly-49D or Ly-49H results in cellular activation, as assessed by induction of tyrosine phosphorylation of multiple cellular substrates. |
