| First Author | Silver ET | Year | 2000 |
| Journal | J Immunol | Volume | 165 |
| Issue | 4 | Pages | 1771-81 |
| PubMed ID | 10925254 | Mgi Jnum | J:333709 |
| Mgi Id | MGI:6705203 | Doi | 10.4049/jimmunol.165.4.1771 |
| Citation | Silver ET, et al. (2000) Ly-49P activates NK-mediated lysis by recognizing H-2Dd. J Immunol 165(4):1771-81 |
| abstractText | Little is known regarding the ligand specificity of Ly-49 activating receptor subfamily members expressed by NK cells. A new Ly-49 activating receptor related to Ly-49A in its extracellular domain, designated Ly-49P, was recently cloned from 129 strain mice. We independently cloned an apparent allele of Ly-49P expressed by nonobese diabetic and nonobese diabetes-resistant mouse strain NK cells. We found it to be reactive with the A1 Ab thought to recognize a polymorphic epitope expressed only by the Ly-49A inhibitory receptor of the C57BL/6 strain. Rat RNK-16 cells transfected with Ly-49P mediated reverse Ab-dependent cellular cytotoxicity of FcR-positive target cells, indicating that Ly-49P can activate NK-mediated lysis. We determined that RNK-16 lysis of Con A blasts induced by Ly-49P was MHC dependent, resulting in efficient lysis of H-2Dd-bearing targets. We found that the Dd alpha1/alpha2 domain is required for Ly-49P-mediated RNK-16 activation, as determined by exon shuffling and transfection. Thus, Ly-49P is the second activating Ly-49 receptor demonstrated to induce NK cytotoxicity by recognizing a class I MHC molecule. |
