| First Author | Seth R | Year | 1993 |
| Journal | Dev Biol | Volume | 158 |
| Issue | 2 | Pages | 555-9 |
| PubMed ID | 8344470 | Mgi Jnum | J:13168 |
| Mgi Id | MGI:61376 | Doi | 10.1006/dbio.1993.1213 |
| Citation | Seth R, et al. (1993) Role of epidermal growth factor expression in early mouse embryo lung branching morphogenesis in culture: antisense oligodeoxynucleotide inhibitory strategy. Dev Biol 158(2):555-9 |
| abstractText | Epidermal growth factor (EGF) expression and branching morphogenesis were inhibited using a 5' 15-mer antisense oligodeoxynucleotide (ODN) directed against EGF precursor mRNA in embryonic mouse lung in culture under chemically defined, serumless conditions. Antisense EGF ODN resulted in > 90% inhibition of EGF immunoreactive peptide synthesis, 75% reduction in branching morphogenesis, 73% decrease in DNA content, 64% decrease in RNA content, 73% decrease in protein synthesis, and 65% decrease in [3H]thymidine incorporation into DNA compared to Embryonic Day 11 controls in culture for 4 days. Sense ODN results were similar to control. Supplementing antisense ODN with EGF partially reversed antisense effects. The results further support a role for EGF in pulmonary organogenesis. |
