Primary Identifier | MGI:3028279 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Npy5r |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | 760bp from the coding region were replaced with lacZ and a neomycin resistance gene. The replaced sequence included the translation initiation codon and sequence coding for the first 5 transmembrane spanning domains. RT-PCR indicated that less than 10% of normal transcript levels were present and this was most likely the consequence of read-through from the neomycin resistance gene rather than wild-type transcript. |