| First Author | Machida H | Year | 2001 |
| Journal | Biochim Biophys Acta | Volume | 1517 |
| Issue | 3 | Pages | 424-9 |
| PubMed ID | 11342221 | Mgi Jnum | J:69997 |
| Mgi Id | MGI:2135929 | Doi | 10.1016/s0167-4781(00)00285-2 |
| Citation | Machida H, et al. (2001) Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene. Biochim Biophys Acta 1517(3):424-9 |
| abstractText | We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element. |
