| First Author | Lin CC | Year | 1980 |
| Journal | Can J Genet Cytol | Volume | 22 |
| Issue | 1 | Pages | 103-16 |
| PubMed ID | 7388680 | Mgi Jnum | J:6332 |
| Mgi Id | MGI:54808 | Doi | 10.1139/g80-013 |
| Citation | Lin CC, et al. (1980) Porcine (Sus scrofa domestica) chromosome identification and suggested nomenclature. Can J Genet Cytol 22(1):103-16 |
| abstractText | Various banding techniques have been used for chromosome analysis in domestic pigs (Sus scrofa domestica). The techniques used in karyotype analysis were Q-banding by (CMA)2S, trypsin-G-banding, BrdU-Acridine-Orange R-Banding and C-banding. Sequential staining techniques of quinacrine-Giemsa were used to record the length of each chromosome and determine arm ratios. Sequential Quinacrine-Giemsa-Ag-AS treatment was used to locate the nucleolar organizer (NOR) on specific chromosomes. A G-C specific fluorochrome was used for reverse fluorescent banding and to differentiate certain chromosome regions which may contain G + C rich DNA. Unequivocal identification of all individual autosomes and sex-chromosomes in the porcine complement is now possible. The X-chromosome of the species has a banding pattern similar to the human X-chromosome. A nomenclature system similar to that used for human chromosomes is proposed for the G-banded and Q-banded karyotype of the domestic pig. The results of C-banding and olivomycin fluorescent banding suggest that at least three types of heterochromatin are contained in the porcine genome. |
