| First Author | Kimura K | Year | 2005 |
| Journal | FEBS Lett | Volume | 579 |
| Issue | 13 | Pages | 2926-32 |
| PubMed ID | 15890343 | Mgi Jnum | J:99788 |
| Mgi Id | MGI:3583840 | Doi | 10.1016/j.febslet.2005.04.042 |
| Citation | Kimura K, et al. (2005) Functional analysis of transcriptional repressor Otx3/Dmbx1. FEBS Lett 579(13):2926-32 |
| abstractText | Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1. |
