| First Author | Poudel B | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 434 |
| Issue | 3 | Pages | 584-8 |
| PubMed ID | 23583376 | Mgi Jnum | J:201275 |
| Mgi Id | MGI:5512920 | Doi | 10.1016/j.bbrc.2013.03.118 |
| Citation | Poudel B, et al. (2013) Induction of IL-12 production by the activation of discoidin domain receptor 2 via NF-kappaB and JNK pathway. Biochem Biophys Res Commun 434(3):584-8 |
| abstractText | We investigated the mechanism involving discoidin domain receptor 2 (DDR2) mediated production of interleukin 12 (IL-12). When compared to control, collagen I upregulated the IL-12 luciferase activity on DDR2 expressing cells. Collagen I induced the phosphorylation of DDR2 and enhanced the phosphorylation of mitogen activated protein kinase (MAPK) kinases. In addition, NF-kappaB binding activity was enhanced when the cells expressing NF-kappaB reporter were exposed to collagen I. Moreover, when IL-12 reporter transfected cells were treated with biochemical inhibitors of c-Jun N-terminal kinase (JNK) and NF-kappaB, collagen-induced IL-12 promoter activity was significantly downregulated in comparison to non-treated cells. Similarly, confirmatory experiments on murine dendritic cells revealed that IL-12 promoter activity is dose dependently downregulated upon NF-kappaB and JNK inhibitor treatment on collagen I stimulation. In summary, DDR2 is involved in the collagen I-induced IL-12 production via NF-kappaB and JNK pathway. |
