| First Author | Lino CNR | Year | 2017 |
| Journal | Eur J Immunol | Volume | 47 |
| Issue | 6 | Pages | 970-981 |
| PubMed ID | 28386934 | Mgi Jnum | J:247185 |
| Mgi Id | MGI:5924555 | Doi | 10.1002/eji.201646753 |
| Citation | Lino CNR, et al. (2017) Eomes expression reports the progressive differentiation of IFN-gamma-producing Th1-like gammadelta T cells. Eur J Immunol 47(6):970-981 |
| abstractText | The transcription factor Eomesodermin (Eomes) plays a crucial role in regulating cytotoxic function, development, and survival of immune cells. gammadelta T cells can express Eomes, but its contribution to their differentiation is unknown. Using Eomes-IRES-GFP mice, we show that Eomes+ gammadelta T cells are unequally distributed among organs, with the highest proportion in spleen. While the majority of Eomes+ gammadelta T cells expressed Vgamma1+ and Vgamma4+ TCRs, Eomes was absent in Vgamma5+ , Vgamma6+ , and Vgamma7+ subsets. Moreover, Eomes was co-expressed in gammadelta T cells with Th1 lineage-related factors such as CD27, T-bet, and Ly6C, but not with Th17 lineage-related genes. Eomes+ and Eomes- gammadelta T-cell populations showed distinct gene expression profiles, with an increase of cytotoxic-related genes in Eomes+ gammadelta T cells. Furthermore, Eomes could be induced in peripheral gammadelta T cells by IL-12 and IL-4, and Eomes+ gammadelta T cells presented a higher proliferation rate and IFN-gamma production when stimulated in vitro with IL-12 and IL-18. However, gammadelta T cells with very high Eomes levels displayed an exhausted phenotype with high levels of PD-1, and were less capable of IFN-gamma production. Together, this study highlights Eomes as a marker for the differentiation of Th1-like effector gammadelta T cells. |
