| Primary Identifier | MGI:1888379 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ptprcap |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| description | NOTE: In contrast to the findings reported here, Matsua et al. (J:48387) report that T and B lymphocytes isolated from Ptprcaptm1Tkda gene-targeted mice fail to respond as well as wild-type to antigen receptor stimulation, and display a diminished association between PTPRC and lymphocyte protein tyrosine kinase (Lck). |
| molecularNote | The entire coding region (exon 2), with the exception of the initiator methionine, was replaced with a neomycin selection cassette. The cassette was inserted in the same transcriptional orientation as the gene in order to preserve the expression of an adjacent loci encoding coronin. Immunoblot analysis showed an absence of encoded protein in lysates derived from the thymi and spleens of homozygous mutant mice. Additional analysis showed that coronin protein levels were not affected by the targeting event. |
