| First Author | Reyes de Mochel NS | Year | 2015 |
| Journal | Dev Biol | Volume | 397 |
| Issue | 1 | Pages | 45-55 |
| PubMed ID | 25446538 | Mgi Jnum | J:218167 |
| Mgi Id | MGI:5616943 | Doi | 10.1016/j.ydbio.2014.10.001 |
| Citation | Reyes de Mochel NS, et al. (2015) BMP signaling is required for cell cleavage in preimplantation-mouse embryos. Dev Biol 397(1):45-55 |
| abstractText | The mechanisms regulating cell division during development of the mouse pre-implantation embryo are poorly understood. We have investigated whether bone morphogenetic protein (BMP) signaling is involved in controlling cell cycle during mouse pre-implantation development. We mapped and quantitated the dynamic activities of BMP signaling through high-resolution immunofluorescence imaging combined with a 3D segmentation method. Immunostaining for phosphorylated Smad1/5/8 shows that BMP signaling is activated in mouse embryos as early as the 4-cell stage, and becomes spatially restricted by late blastocyst stage. Perturbation of BMP signaling in preimplantation mouse embryos, whether by treatment with a small molecule inhibitor, with Noggin protein, or by overexpression of a dominant-negative BMP receptor, indicates that BMPs regulate cell cleavage up to the morula stage. These results indicate that BMP signaling is active during mouse pre-implantation development and is required for cell cleavage in preimplantation mouse embryos. |
