| First Author | Boehm B | Year | 2011 |
| Journal | Development | Volume | 138 |
| Issue | 6 | Pages | 1227-34 |
| PubMed ID | 21307091 | Mgi Jnum | J:170492 |
| Mgi Id | MGI:4946572 | Doi | 10.1242/dev.057547 |
| Citation | Boehm B, et al. (2011) A landmark-free morphometric staging system for the mouse limb bud. Development 138(6):1227-34 |
| abstractText | We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about +/-2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns. |
