| First Author | Chun KR | Year | 2009 |
| Journal | Cell Immunol | Volume | 256 |
| Issue | 1-2 | Pages | 92-8 |
| PubMed ID | 19246034 | Mgi Jnum | J:148553 |
| Mgi Id | MGI:3845701 | Doi | 10.1016/j.cellimm.2009.01.011 |
| Citation | Chun KR, et al. (2009) Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells. Cell Immunol 256(1-2):92-8 |
| abstractText | The molecular action mechanism of MRP, one of the protein kinase C (PKC) substrates, has been under intense investigation, but reports on its role in macrophage function remain controversial. The treatment of macrophage cell lines with bacterial lipopolysaccharide (LPS) induced a high level of MRP expression suggesting that MRP plays a role in the function of activated macrophages. In order to investigate the role of MRP in activated RAW264.7 cells, we stably transfected MRP-specific shRNA expression constructs and tested for alterations in macrophage-related functions. The down-regulation of MRP expression resulted in a marked reduction in chemotaxis toward MCP-1 or extracellular matrix proteins. Furthermore, pharmacological inhibitors of PKC significantly inhibited the chemotaxis in RAW264.7 cells. These data reveals the pivotal role of MRP in the transmigration of activated RAW264.7 cells. |
