Primary Identifier | MGI:7442594 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(Atoh1-lacZ)4Jejo |
Strain of Origin | (C57BL/6 x DBA)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | This transgene was created by coinjection of two overlapping genomic fragments: an NcoI-NcoI restriction fragment containing sequence 5' to and 3' from the Atoh1 coding sequence and a BglII-BglII fragment containing the last ~400 bp of coding sequence and 6 kb 3' of the coding region. The coding sequence between the first ~2.5 kb and the last ~1.5 kb was replaced in-frame with the beta-galactosidase reporter gene (without NLS sequence). |