Primary Identifier | MGI:6304325 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Zfp638 |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTCAAGCTGACAAATCAATT and ATTATCACGCTTGTAAAGAA, which resulted in a 136 bp deletion beginning at Chromosome 6 position 83,934,897 bp and ending after 83,935,032 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000194351 (exon 3) and 74 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 437 and early truncation 2 amino acids later. |