Primary Identifier | MGI:6478915 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Brme1 |
Strain of Origin | C57BL/6N | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Exons 3-9 were targeted for deletion with two synthetic crRNAs (targeting GCAGGAAGTTCATAGCCACA(ggg) and TGGGTAACAGATCTACACAC(agg)) and an ssODN (GGCTACAACTACAGGGAGGTTTTGTGCTCTCTAACCTGTGaattcGGCTATGAACTTCCTGCCGC CATCAGCTTCCAAAATACAG) using CRISPR/Cas9 technology. |