| First Author | Ueno H | Year | 2001 |
| Journal | Genomics | Volume | 78 |
| Issue | 1-2 | Pages | 91-8 |
| PubMed ID | 11707077 | Mgi Jnum | J:72605 |
| Mgi Id | MGI:2153299 | Doi | 10.1006/geno.2001.6641 |
| Citation | Ueno H, et al. (2001) Characterization of the Gene EPAC2: Structure, Chromosomal Localization, Tissue Expression, and Identification of the Liver-Specific Isoform. Genomics 78(1/2):91-8 |
| abstractText | The liver-specific protein cAMP-GEFII (also known as Epac2) belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins having guanine nucleotide exchange factor (GEF) activity (the cAMP-GEF family). Here we clone the gene EPAC2, encoding cAMP-GEFII, from a human liver cDNA library. Human EPAC2 has at least 31 exons and is mapped to human chromosome 2q31. Analyses by primer extension, reverse transcriptase-polymerase chain reaction, and in situ hybridization revealed the presence of three transcription start sites of liver-specific Epac2: two major sites located in exon 10 and a minor site in intron 9. The same translation start site is used in all three transcripts. Liver-specific cAMP-GEFII protein, which lacks the first cAMP-binding domain and the Dishevelled/Egl-10/Pleckstrin domain, was detected at 79 kDa by immunoblot analysis, confirming the presence of the short form of cAMP-GEFII in the liver. Liver-specific cAMP-GEFII also has GEF activity toward Rap1. These results demonstrate the presence of liver-specific cAMP-GEFII. Together with the previous finding that cAMP-GEFII is responsible for cAMP-dependent exocytosis in secretory cells, our study suggests that cAMP-GEFII may have a distinct role in liver. |
