| First Author | Rhaissi H | Year | 1993 |
| Journal | Biochimie | Volume | 75 |
| Issue | 10 | Pages | 899-904 |
| PubMed ID | 8312393 | Mgi Jnum | J:16540 |
| Mgi Id | MGI:64613 | Doi | 10.1016/0300-9084(93)90046-u |
| Citation | Rhaissi H, et al. (1993) Multiple leader sequences for mouse cathepsin B mRNA?. Biochimie 75(10):899-904 |
| abstractText | We previously described the gene structure of murine cathepsin B. Our results suggested that the 5'-untranslated region (leader) is interrupted by a large intron. The second exon (exon-2) contains the translation initiation site. To characterize the leader region, a rapid amplification of cDNA ends (RACE) procedure was developed. The PCR products were directly cloned and sequenced. Nucleotide sequence analyses revealed three different 5'-cDNA ends, suggesting the existence of three different leader regions. In addition to the leader (LA) previously characterized, we now describe two other 5'-untranslated regions, LB and LC. Leader LB is located 2.3 kb upstream exon-2, and leader LC corresponds to the 3'-end of the first intron and is thus contiguous to exon-2. Our results suggest for murine cathepsin B gene the presence of multiple promoters, and possibly the expression of multiple mRNAs differing in their leader region. |
