First Author | Biris KK | Year | 2014 |
Journal | Methods Mol Biol | Volume | 1092 |
Pages | 17-30 | PubMed ID | 24318811 |
Mgi Jnum | J:210109 | Mgi Id | MGI:5569570 |
Doi | 10.1007/978-1-60327-292-6_2 | Citation | Biris KK, et al. (2014) Two-color in situ hybridization of whole-mount mouse embryos. Methods Mol Biol 1092:17-30 |
abstractText | RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos. |