| First Author | Wang P | Year | 2004 |
| Journal | Cell Mol Life Sci | Volume | 61 |
| Issue | 18 | Pages | 2405-17 |
| PubMed ID | 15378209 | Mgi Jnum | J:218001 |
| Mgi Id | MGI:5616324 | Doi | 10.1007/s00018-004-4256-z |
| Citation | Wang P, et al. (2004) Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines. Cell Mol Life Sci 61(18):2405-17 |
| abstractText | Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20 degrees C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation. |
