| First Author | Lewis GM | Year | 2016 |
| Journal | J Clin Invest | Volume | 126 |
| Issue | 10 | Pages | 3799-3813 |
| PubMed ID | 27599295 | Mgi Jnum | J:237280 |
| Mgi Id | MGI:5811938 | Doi | 10.1172/JCI87041 |
| Citation | Lewis GM, et al. (2016) TGF-beta receptor maintains CD4 T helper cell identity during chronic viral infections. J Clin Invest 126(10):3799-3813 |
| abstractText | Suppression of CD8 and CD4 T cells is a hallmark in chronic viral infections, including hepatitis C and HIV. While multiple pathways are known to inhibit CD8 T cells, the host molecules that restrict CD4 T cell responses are less understood. Here, we used inducible and CD4 T cell-specific deletion of the gene encoding the TGF-beta receptor during chronic lymphocytic choriomeningitis virus infection in mice, and determined that TGF-beta signaling restricted proliferation and terminal differentiation of antiviral CD4 T cells. TGF-beta signaling also inhibited a cytotoxic program that includes granzymes and perforin expression at both early and late stages of infection in vivo and repressed the transcription factor eomesodermin. Overexpression of eomesodermin was sufficient to recapitulate in great part the phenotype of TGF-beta receptor-deficient CD4 T cells, while SMAD4 was necessary for CD4 T cell accumulation and differentiation. TGF-beta signaling also restricted accumulation and differentiation of CD4 T cells and reduced the expression of cytotoxic molecules in mice and humans infected with other persistent viruses. These data uncovered an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-beta signaling. During chronic viral infection, this program limits CD4 T cell responses while maintaining CD4 T helper cell identity. |
