| First Author | Lomedico PT | Year | 1984 |
| Journal | Nature | Volume | 312 |
| Issue | 5993 | Pages | 458-62 |
| PubMed ID | 6209582 | Mgi Jnum | J:7660 |
| Mgi Id | MGI:56129 | Doi | 10.1038/312458a0 |
| Citation | Lomedico PT, et al. (1984) Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature 312(5993):458-62 |
| abstractText | Interleukin-1 (IL-1), a peptide hormone produced by activated macrophages, possesses the ability to modulate the proliferation, maturation and functional activation of a broad spectrum of cell types and may play a major role in the initiation and amplification of immune and inflammatory responses through its action on these diverse cell populations. IL-1 exhibits microheterogeneity in terms of its relative molecular mass (Mr, 13,000-19,000) and charge properties, and although murine IL-1 has been purified and some of its basic structure-function relationships have been elucidated, it has proved difficult to prepare sufficient amounts of IL-1 for direct and detailed sequence and structural studies. Here we report the cloning, sequence analysis and expression of murine IL-1 cDNA in Escherichia coli. The IL-1 cDNA codes for a polypeptide precursor of 270 amino acids. Biologically active IL-1 was produced in E. coli by expressing the carboxy-terminal 156 amino acids of the IL-1 precursor. |
