| First Author | Raymond CS | Year | 2007 |
| Journal | PLoS One | Volume | 2 |
| Issue | 1 | Pages | e162 |
| PubMed ID | 17225864 | Mgi Jnum | J:128069 |
| Mgi Id | MGI:3766051 | Doi | 10.1371/journal.pone.0000162 |
| Citation | Raymond CS, et al. (2007) High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS One 2(1):e162 |
| abstractText | DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre. |
