| First Author | Yamada G | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 199 |
| Issue | 2 | Pages | 552-63 |
| PubMed ID | 7907867 | Mgi Jnum | J:17179 |
| Mgi Id | MGI:65230 | Doi | 10.1006/bbrc.1994.1264 |
| Citation | Yamada G, et al. (1994) Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies. Biochem Biophys Res Commun 199(2):552-63 |
| abstractText | Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed. |
