Primary Identifier | MGI:6712744 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Eomes |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct replaced about 500 bp of the coding sequence in exon 1 with a membrane-localized tdTomato reporter gene (mTomato), a T2A sequence, nuclear-localized GFP (H2B:GFP), and polyadenylation sequence (bGH PolyA). Cre-mediated recombination removed the floxed neomycin selection cassette introduced downstream of the inserted sequence. |