| First Author | Robert T | Year | 2016 |
| Journal | Science | Volume | 351 |
| Issue | 6276 | Pages | 943-9 |
| PubMed ID | 26917764 | Mgi Jnum | J:229524 |
| Mgi Id | MGI:5752405 | Doi | 10.1126/science.aad5309 |
| Citation | Robert T, et al. (2016) The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science 351(6276):943-9 |
| abstractText | Meiotic recombination is induced by the formation of DNA double-strand breaks (DSBs) catalyzed by SPO11, the ortholog of subunit A of TopoVI DNA topoisomerase (TopoVIA). TopoVI activity requires the interaction between A and B subunits. We identified a conserved family of plant and animal proteins [the TOPOVIB-Like (TOPOVIBL) family] that share strong structural similarity to the TopoVIB subunit of TopoVI DNA topoisomerase. We further characterize the meiotic recombination proteins Rec102 (Saccharomyces cerevisiae), Rec6 (Schizosaccharomyces pombe), and MEI-P22 (Drosophila melanogaster) as homologs to the transducer domain of TopoVIB. We demonstrate that the mouse TOPOVIBL protein interacts and forms a complex with SPO11 and is required for meiotic DSB formation. We conclude that meiotic DSBs are catalyzed by a complex involving SPO11 and TOPOVIBL. |
