Primary Identifier | MGI:4352684 | Allele Type | Targeted |
Gene | Myc | Transmission | Germline |
Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
Is Wild Type | false |
molecularNote | A cassette containing 25 tandem I-SceI target sites (S. cerevisiae) endonuclease recognition site (TAGGGATAACAGGGTAAT) was inserted into intron 1 of the gene. A floxed neomycin cassette was included upstream of the I-SceI cassette and was subsequently removed by transient expression of cre recombinase. Correct targeting was confirmed by Southern blotting. |