| First Author | Tang Y | Year | 2010 |
| Journal | Genesis | Volume | 48 |
| Issue | 9 | Pages | 563-7 |
| PubMed ID | 20645309 | Mgi Jnum | J:170016 |
| Mgi Id | MGI:4943803 | Doi | 10.1002/dvg.20654 |
| Citation | Tang Y, et al. (2010) The contribution of the Tie2+ lineage to primitive and definitive hematopoietic cells. Genesis 48(9):563-7 |
| abstractText | The regulatory elements of the Tie2/Tek promoter are commonly used in mouse models to direct transgene expression to endothelial cells. Tunica intima endothelial kinase 2 (Tie2) is also expressed in hematopoietic cells, although this has not been fully characterized. We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26R-EYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subclasses of early hematopoietic progenitors, T cells, monocytes, granulocytes, and B cells. We found that approximately 84% of each lineage was EYFP(+), and nearly all cells that come from Tie2-expressing lineages are CD45(+), confirming widespread contribution to definitive hematopoietic cells. In addition, more than 82% of blood cells within the embryonic yolk sac were of Tie2(+) origin. Our findings of high levels of Tie2-Cre recombination in the hematopoietic lineage have implications for the use of the Tie2-Cre mouse as a lineage-restricted driver strain. |
