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Allele : Tg(Chat-flpo)22Majh transgene insertion 22, Marthe Howard
Primary Identifier  MGI:6387001 Allele Type  Transgenic
Attribute String  Recombinase Gene  Tg(Chat-flpo)22Majh
Strain of Origin  C57BL/6 x SJL Is Recombinase  true
Is Wild Type  false
molecularNote  The 176 kb bacterial artificial chromosome, RP24-256F2, containing the entire Chat and Slc18a3 (VAChT) genes was modified to insert an optimized FLP recombinase (FLPo) followed by a roxP-flanked PGK-prokaryotic gb2 promoter-neoR/kanR cassette under the direction of the Chat promoter. Induction of Dre recombinase removes the kanamycin cassette in recombineering competent bacteria. Next, a 3X Stop is inserted into the Slc18a3 (or VAChT) blocking transcription.
  • mutations:
  • Insertion
  • synonyms:
  • ChAT-FlpO,
  • ChAT-FlpO
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