First Author | Suh JG | Year | 1997 |
Journal | Mol Cells | Volume | 7 |
Issue | 2 | Pages | 204-7 |
PubMed ID | 9163733 | Mgi Jnum | J:40537 |
Mgi Id | MGI:700177 | Citation | Suh JG, et al. (1997) Sequence analysis, tissue expression and chromosomal localization of a mouse secreted superoxide dismutase gene. Mol Cells 7(2):204-7 |
abstractText | Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells. |