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Publication : Sequence analysis, tissue expression and chromosomal localization of a mouse secreted superoxide dismutase gene.

First Author  Suh JG Year  1997
Journal  Mol Cells Volume  7
Issue  2 Pages  204-7
PubMed ID  9163733 Mgi Jnum  J:40537
Mgi Id  MGI:700177 Citation  Suh JG, et al. (1997) Sequence analysis, tissue expression and chromosomal localization of a mouse secreted superoxide dismutase gene. Mol Cells 7(2):204-7
abstractText  Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.
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