| First Author | Beckstead WA | Year | 2008 |
| Journal | Mamm Genome | Volume | 19 |
| Issue | 10-12 | Pages | 687-90 |
| PubMed ID | 18958524 | Mgi Jnum | J:144051 |
| Mgi Id | MGI:3829830 | Doi | 10.1007/s00335-008-9149-2 |
| Citation | Beckstead WA, et al. (2008) SNP2RFLP: a computational tool to facilitate genetic mapping using benchtop analysis of SNPs. Mamm Genome 19(10-12):687-90 |
| abstractText | Genome-wide analysis of single nucleotide polymorphism (SNP) markers is an extremely efficient means for genetic mapping of mutations or traits in mice. However, this approach often defines a relatively large recombinant interval. To facilitate the refinement of this interval, we developed the program SNP2RFLP. This program can be used to identify region-specific SNPs in which the polymorphic nucleotide creates a restriction fragment length polymorphism (RFLP) that can be readily assayed at the benchtop using restriction enzyme digestion of SNP-containing PCR products. The program permits user-defined queries that maximize the informative markers for a particular application. This facilitates fine-mapping in a region containing a mutation of interest, which should prove valuable to the mouse genetics community. SNP2RFLP and further details are publicly available at http://genetics.bwh.harvard.edu/snp2rflp/ . |
