First Author | Moss SE | Year | 1988 |
Journal | Eur J Biochem | Volume | 177 |
Issue | 1 | Pages | 21-7 |
PubMed ID | 2972541 | Mgi Jnum | J:14450 |
Mgi Id | MGI:62618 | Doi | 10.1111/j.1432-1033.1988.tb14340.x |
Citation | Moss SE, et al. (1988) Molecular cloning of murine p68, a Ca2+-binding protein of the lipocortin family. Eur J Biochem 177(1):21-7 |
abstractText | A plasmid cDNA library prepared from a T-lymphocyte clone of murine strain B10.A origin was screened by cross-species DNA hybridisation using a partial human p68 cDNA clone, identified as containing coding sequences for previously determined amino-acid sequences. The longest p68 cDNA insert from this library and a full-length cDNA insert from a second similar library were fully sequenced. A comparison of the derived amino-acid sequence with that of human p68 revealed extensive homology (95% overall). Homology at the nucleotide level was 89% in the open reading frame and 85% and 50% in the 5' (33 nucleotides) and 3' (347 nucleotides) non-coding regions respectively. Eight segments of internal homology were observed, each containing a highly conserved consensus region of 17 amino acids correlating with that described for several membrane associated calcium-binding proteins [Geisow, M. J., Fritsche, U., Hexham, J. M. & Johnson, T. (1986) Nature (Lond.) 320, 636-638]. These results provide further evidence that p68 is a member of the same gene family as p32,p36 and lipocortin I and demonstrate an unusually high level of inter-species sequence conservation of p68 between mouse and human. |