Primary Identifier | MGI:6324571 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Mir204 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | IRES-lacZ reporter cassette as wells as a loxP site were inserted 178 bp upstream miR-204 stem loop and a loxP site plus a neomycin selection cassette were placed 260 bp downstream miRNA stem loops by homologous recombination in ES cells. The chimeric mice where crossed to flippase-expressing transgenic mice to remove Fret or F3 (a mutant of Frt) flanked lacZ and Neo cassettes, leaving two loxP sites immidiately flanking the miRNA stem-loops. |