| First Author | Maxwell PH | Year | 1993 |
| Journal | Kidney Int | Volume | 44 |
| Issue | 5 | Pages | 1149-62 |
| PubMed ID | 8264149 | Mgi Jnum | J:128415 |
| Mgi Id | MGI:3767088 | Doi | 10.1038/ki.1993.362 |
| Citation | Maxwell PH, et al. (1993) Identification of the renal erythropoietin-producing cells using transgenic mice. Kidney Int 44(5):1149-62 |
| abstractText | Regulation of erythropoietin production by the kidneys is central to the control of erythropoiesis. Uncertainty about the identity of the renal cells involved has been a major obstacle to understanding this mechanism. We have used sequence from the mouse erythropoietin locus to direct expression of a marker gene, SV40 T antigen, to these cells in transgenic mice. The transgenic constructs contained an oligonucleotide marker (Epo-M) or SV40 sequence (Epo-TAg) in the 5' untranslated region of the mouse erythropoietin gene, flanked on each side by 9 and 7.5 kb of DNA from the mouse erythropoietin locus. Anemia-inducible expression of Epo-M and Epo-TAg was observed in the kidney. In one of thirteen lines, homologous integration of Epo-TAg into the mouse erythropoietin locus occurred. In transgenic mice bearing Epo-TAg at homologous and heterologous insertion sites, renal expression was restricted to a population of cells in the interstitium of the cortex and outer medulla. Immunohistochemical characterization by light and electron microscopy shows that these are the fibroblast-like type I interstitial cells. |
